Human Fibrinogen Heterogeneities

Fibrinogen Binding to Human Platelets*

Platelet-activating factor 1-0-alkyl-2-acetyl-englycero-3-phosphocholine (PAF-acether) triggers exposure of fibrinogen binding sites on platelets via binding to specific receptors. Comparison of 13H]PAFacether binding with lZ8I-fibrinogen binding shows that the rate with which PAF-acether binds to a number of receptors and not the degree of receptor occupancy determines how much fibrinogen bin...

Biosynthesis of Human Fibrinogen SUBUNIT

Stable transfected baby hamster kidney (BHK) cells expressing human a, 8, and y fibrinogen chains together, in various combinations of any two, or individually, were established. Several types of subunit interactions were observed in the intracellular extracts of the transfected BHK cell lines as well as in Hep G2 cells. These included: 1) formation of ay dimers linked by a disulfide bond(s), 2...

Crystal structure of human fibrinogen.

A crystal structure of human fibrinogen has been determined at approximately 3.3 A resolution. The protein was purified from human blood plasma, first by a cold ethanol precipitation procedure and then by stepwise chromatography on DEAE-cellulose. A product was obtained that was homogeneous on SDS-polyacrylamide gels. Nonetheless, when individual crystals used for X-ray diffraction were examine...

Veraseal, INN-human fibrinogen / human thrombin

The purification of human fibrinogen.

For investigations concerning the mode of action of the clearing factor, estimations of free fatty acids in mixtures of normal rat plasma and lipaemic dog plasma were performed using a modification of van de Kamer's (1948) method as described for faeces. Because the method developed may be of value for similar or other uses, it is given here. In a 100 ml. flask 2 ml. of the plasma-mixture, cont...